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Product Description | The rabbit anti-acetyl lysine antibody (anti-AcK) is developed using acetylated KLH as the immunogen. It is affinity purified with acetyl lysine agarose, followed by conjugation with horseradish peroxidase(HRP). | Western blot analysis of acetylated histone from TSA-treated (A) and non-treated (B) melanoma cells using ICP0381. | |
Species | Rabbit | ||
Formulation | 250 µg/mL in stabilizing buffer with 50% glycerol | ||
Immunogen | Acetylated KLH conjugates | ||
Purification | Affinity chromatography with acetyl-lysine agarose | ||
Conjugation | Reductive amination; HRP:Ab molar ratio =10:1 | ||
Specificity | The antibody recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA and acetylated MBP. No cross-reaction to the non-acetylated proteins. | ||
Applications | For direct immunofluorescence experiments | ||
Scientific Description | DNA transcription cannot take place unless DNA is unwound from the nucleosomes. The cell unwinds the DNA by acetylating the lysine residues within the proteins. It has been suggested that acetylation of non-histone proteins (e.g. transcription factors) and histones may be involved. Acetylation of the proteins may result in signal transduction within the chromatic domains. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly. | ||
Product Specific References |
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加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化