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Product Description | This antibody was developed using glutathione (GSH)-KLH conjugates and affinity purified with antigen-specific affinity chromatography. The antibody could be utilized for detection and quantization of reduced (GSH) and oxidized (GSSG) glutathione as well as glutathionylated proteins and peptides. | Western blot analysis of the glutathionylated proteins in MMRU cell lysates. Right lane: sample boiled with DTT. IF staining of the MMRU cells (5 µg/mL). | |
Species | Rabbit | ||
Formulation | 250 µg/mL in PBS with 50% glycerol | ||
Immunogen | Glutathione-KLH conjugates linked through -S- bonds | ||
Purification | Antigen-specific affinity chromatography | ||
Specificity | The pan-specific antibodies recognize both GSH and GSSG as well as glutathionylated proteins and peptides. | ||
Applications | Quantitative ELISA; WB; IP (affinity chromatography); IF; IHC | ||
Scientific Description | Glutathione is a tripeptide. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. Glutathione, an antioxidant, protects cells from toxins such as free radicals. Glutathione can also modify proteins via disulfide bonds. Redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes have been reported. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored. | ||
Product Specific References | 1. PNAS.2005. 102(39): 13998-14003. doi:10.1073/pnas.05043981022. J. Biol. Chem.2007. 282(45): 32640-54.doi:10.1074/jbc.M702953200 |
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化