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Product Description | Crebtide (KRREILSRRPSYR) is a substrate peptide derived from the protein Creb which is widely utilized as a synthetic substrate for the PKA and PKC kinase families. Affinity purified rabbit polyclonal anti-pCrebtide is developed with phosphorylated Crebtide (KRREILSRRPpSYR) and absorbed with non-phosphorylated Crebtide. | Western-blot analysis of the BSA-Crebtide phosphorylated with PKC alpha Kinase/assay Western-blot analysis of the BSA-Crebtide phosphorylated with PKC alpha Kinase/assay: A: 0.0 ng B: 3 ng C: 6 ng D: 12.5 ng E: 25 ng F: 50 ng Western-blot analysis of the BSA-Crebtide phosphorylated with PKC alpha Kinase/assay: A:0.0 ng B:4 ng C:15 ng D:60 ng E:250 ng. | |
Species | Rabbit | ||
Formulation | 250 µg/mL in TBS, 0.1% NaN3 | ||
Immunogen | Phospho-Crebtide (KRREILSRRPpSYR)-KLH | ||
Purification | The antibody was affinity purified with KRREILSRRPpSYR on agarose followed by affinity desorption with non-phosphorylated Crebtide. | ||
Specificity | The antibody recognizes only the phosphorylated Crebtide product of PKA and PKC in a non-radioactive kinase assay. There are no cross-reactions with the non-phosphorylated Crebtide. | ||
Applications | ELISA; WB; Non-radioactive PKA and PKC kinase assay | ||
Scientific Description | p33 (ING1) is a new growth inhibitor candidate for a tumour suppressor gene. p33 (ING1) cooperates with p53 and down-regulates cell growth by activating transcription from the p21/WAF1 promoter although there is no sequence similarity between p33 (ING1) and p53. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if stored properly. | ||
Product Specific References | 1. J. Biol. Chem. 1971. 246(18): 5770-5784. |
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化