| Product Description | A high density of the acetylated lysine antibody is immobilized to agarose beads. The product can be utilized as an affinity matrix for rapid isolation and purification of proteins/peptides with acetyl lysine residues. | | The acetylated protein signal as determinated by ELISA titration (OD50 titer). The acetlated proteins were eluted from 1 mL the Rabbit anti-Acetyllysine Agarose (ICP0388) and immobilized on microrplate, followed by detection with Mouse anti-Acetylated lysine HRP. Rabbitt anti-Biotin Agarose (1 mL) was utilized as the negative control. The maximum binding of acetylated BSA with ICP0388-5MG. -50 µl of ICP0388-5MG were incubated with 1 mg of acetylate BSA in a 1 ml tube for 60 min. - After washing with PBST 4 times, the bound acetylated BSA was eluted with 1 ml of 0.5 M HCl -5 µl / lane of the eluted acetylated BSA was resolved by SDS-PAGE and blotted wih monoclonal Mouse anti-Acetylated Lysine (ICP0390) -ECL exposure 3 seconds |
| Formulation | 0.5 mL of agarose beads suspended in 1 mL of slurry |
| Antibody Immobilized | 10 mg/mL antibody is covalently linked through amide bonds with NHS activated-SMCC, which further conjugates to thiolated agarose beads via thiol ether bonds. |
| Specificity | This antibody affinity matrix selectively captures proteins/peptides with acetylated lysine residues (N-epsilon). No cross creation to methylated proteins/peptides. |
| BindingCapacity | Approximately 0.5-1 mg of acetylated histone / mL slurry. |
| Applications | Rapid isolation and purification of proteins/peptides with acetylated lysine residues from cell lysate or protease-digested mixtures. |
| Scientific Description | Protein acetylation is a form of post-translational modification known to regulate many diverse biological processes. Detection, isolation and identification of acetylated proteins/peptides is essential in proteomic studies. Affinity chromatography is one of the most efficient and rapid methods to enrich and purify the acetylated species for further MS/MS identification. |
| Storage & Stability | Product is stable for 30 days at room temperature. For extended storage, store product at -20°C. Do not aliquot and shake thoroughly before use.Expiration date is one year from date of shipping if properly stored. |
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