商品介绍
Mouse IgG Antibody, Agarose
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Product Description | Monoclonal antibody production using cell culture systems sometimes requires the use of fetal calf serum. Purification of the mouse antibody from the cell culture supernatant using Protein A/G systems may result in contamination with bovine IgG. Anti-mouse IgG agarose is the mouse IgG-specific affinity matrix that could selectively purify mouse IgG from mouse serum, both ascites and the supernatant of the cell culture media, without pulling-down bovine IgG. This anti-mouse IgG agarose is prepared using antigen-specific affinity purified anti-mouse IgG as the immobilized antibodies. More than 20 mg of pure anti-mouse IgG was covalently immobilized onto the agarose beads via a highly stable thiol-ether bond. This product has been successfully used to replace Protein A/G agarose for more specific affinity purification of mouse IgG with high binding capacity (> 10 mg/mL). | |
Formulation | 1 mL beads in 2 mL 50% glycerol | |
| Binding Capacity | >10 mg/mL of mouse IgG | |
Applications | Affinity chromatography ; Immunoprecipitation | |
| Ligand Immobilized | 40 mg anti-mouse IgG/mL | |
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. | |
| Product Specific References |
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Purification of mouse IgG from monoclonal cell culture supernatant with anti-mouse IgG agarose. Fraction 1: 0.8 mg/mL Fraction 2: 2.5 mg/mL IgG Std: 0.31 - 5 mg/mL |  | |
品牌介绍
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供，并均已获得了技术持有人（公司）的授权。 主营产品公司提供以下产品： 1.细胞信号传导通路抗体，翻译后修饰抗体，细胞凋亡相关抗体，非放射性蛋白激酶测试盒，亲和填料； 2.磷酸化抗体，非磷酸化抗体，甲基化抗体，乙酰化
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