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Product Description | The biotin antibody is developed using biotin-KLH conjugates and purified with biotin-agarose affinity chromatography. The purified biotin antibody was chemically linked to beaded agarose through a stable covalent linkage. The immobilized biotin antibody on beaded agarose could be utilized for reversible immunoprecipitation and affinity purification of both biotinylated antibodies and biotinylated proteins. This product has been applied to the MS-based proteomics experiments as per paper (Udeshi et al in Nature Methods Vol.14 pages: 1167-1170 (2017)). |
Formulation | 1 mL beads in 2 mL slurry with glycerol |
Antibody Immobilized | 10 mg/mL agarose |
Applications | IP; Immuno-affinity chromatography; |
Scientific Description | Biotin is a vitamin, which binds strongly and irreversibly to avidin. Biotin is commonly used for labelling reagents. Anti-biotin antibody binds to biotin strongly but reversibly, the binding of the biotinylated species could be competitively eluted with free biotin. |
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Do not aliquot and shake throughly before use. Expiration date is one year from date of shipping if properly stored. |
Product Specific References |
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加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化