Phosphoserine Antibody
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Product Description | Affinity purified rabbit polyclonal phospho-serine antibodies are developed using a phospho-serine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-serine specific antibodies were affinity purified with serine-phosphorylated peptide immobilized on agarose. This affinity purified antibody showed higher affinity than phospho-serine antibodies from another source. |  Detection of a phosphorylated protein kinase with anti-phosphoserine (ICP9806): Lane1-2: Kinase untreated Lane 3-6: kinase treated with phosphatase PP1G  A: Control B-D: Treatment with increased UV dose E: Phosphatase inhibitor treatment  | |
Species | Rabbit | ||
| Formulation | 250 µg/mL in PBS, 50% glycerol | ||
| Immunogen | Phospho-serine KLH and phosvitin mixture | ||
| Purification | Affinity purified with phospho-tyrosine on agarose | ||
| Specificity | The antibody recognizes proteins and peptides phosphorylated on serine residues. There are no cross reactions with phospho-tyrosine. | ||
| Tested | Human breast cancer tissue and mouse cell | ||
| Applications | ELISA (1:250); WB (1:250); IP (10 µg/mg crude protein); IHC (1:50) | ||
| Scientific Description | Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to phospho-serine which is responsible for various signalling processes in eukaryotes. | ||
| Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. | ||
Product Specific References |
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加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供，并均已获得了技术持有人（公司）的授权。 主营产品公司提供以下产品： 1.细胞信号传导通路抗体，翻译后修饰抗体，细胞凋亡相关抗体，非放射性蛋白激酶测试盒，亲和填料； 2.磷酸化抗体，非磷酸化抗体，甲基化抗体，乙酰化
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