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Product Description | The affinity purified rabbit polyclonal anti-phospho-serine (anti-pS) was developed using phospho-serine-KLH conjugates and a phospho-protein mixture as the immunogen. The phospho-serine specific antibody was affinity purified with serine-phosphorylated peptide immobilized to agarose. The affinity-purified anti-pS was conjugated to peroxidase (HRP) by reductive amination. Direct application of the primary conjugates will avoid non-specific signals, particularly due to the antibody signal on Western blot analysis of IP"s with antibodies from the same species. |
Species | Rabbit |
Formulation | 250 µg/mL in PBS, 50% glycerol |
Immunogen | Phospho-serine-KLH and phosvitin mixture |
Purification | The antibody was purified with phospho-serine on agarose. |
Conjugation | Reductive amination; HRP/Ab molar ratio - 1:10 |
Specificity | The antibody recognizes proteins phosphorylated on serine residues. There are no cross reactions with phospho-tyrosine. |
Applications | ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL) |
Scientific Description | Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to form phospho-serine, which is responsible for various signalling processes in eukaryotes. [1] |
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is six months from date of shipping if properly stored. |
Product Specific References |
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加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化