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Product Description | Affinity purified rabbit polyclonal phospho-threonine antibody (anti-pT) was developed using a phospho-threonine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-threonine specific antibody was affinity purified with phospho-threonine immobilized on agarose. | Detection of a phosphorylated protein kinase with anti-phosphothreonine (ICP9807) Lane1: Untreated kinase Lane 2: Kinase treated with phosphatase PP1G Lane 3: MW marker time course Western blot analysis of mouse extract spleen with ICP9807 A: Extract of spleen from mouse treated with sodium vanadate B: Extract of spleen from control mouse | |
Species | Rabbit | ||
Formulation | 250 µg/mL in PBS, pH 7, with 50% glycerol | ||
Immunogen | Phospho-threonine KLH conjugates and phosvitin mixture | ||
Purification | The antibody was affinity purified with phospho-threonine on agarose followed by affinity absorption with threonine on agarose. | ||
Specificity | The antibody recognizes proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phospho-tyrosine. | ||
Species Tested | Human and mouse | ||
Applications | ELISA; WB; IP | ||
Scientific Description | Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to phospho-threonine which enters various biosynthetic pathways in the body. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. | ||
Product Specific References |
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加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化