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Product Description | Affinity purified rabbit anti-phospho-threonine antibodies are conjugated to d-biotin via N-acetylation. The conjugates are useful for detection, identification and quantization of threonine-phosphorylated proteins and peptides. |
Species | Rabbit |
Formulation | 250 µg/mL in PBS, 50% glycerol |
Immunogen | Phospho-threonine-KLH conjugates and phosvitin |
Purification | The antibody was purified on phospho-threonine agarose. |
Conjugation | Acetylation of the primary amine with the active ester of biotin (NHS-activated d-biotin) |
Specificity | The antibody is pan-specific against phospho-threonine. It cross reacts with phospho-serine slightly but does not cross-react with phospho-tyrosine. |
Applications | ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL) |
Scientific Description | Threonine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate threonine to form phospho-threonine which is considered a key step in the regulation of enzymatic activities such as signal transduction. |
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored. |
Product Specific References |
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加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化