The antibody is raised using the immunogenic conjugate phospho-tyrosine linked to KLH. The pan-specific antibodies for phospho-tyrosine were specifically and selectively isolated from the immune serum using a unique technique which makes sure the antibody can be competitively displaced by free phospho-tyrosine. This pan-specific anti-phospho-tyrosine (anti-pY) antibody could recognize the phospho-tyrosine residues on both proteins and peptides. It has proved to be useful for affinity isolation, localization and detection of tyrosine phosphorylated proteins. This antibody could be utilized for immunoprecipitation and affinity enrichment of tyrosine-phosphorylated proteins in cell and tissue lysates. It could be useful for immunoblotting tyrosine-phosphorylated proteins and ELISA quantization of protein tyrosine phosphorylation. It could also be used for non-radioactive tyrosine kinase assay.

Rabbit

250 µg/mL in PBS, 50% glycerol, pH 7, 0.01% NaN₃

Phospho-tyrosine KLH conjugates

Affinity purified with phospho-tyrosine on agarose and competitively eluted with N-acetylated phospho-tyrosine

The antibody recognizes proteins and peptides phosphorylated on tyrosine residues. There are no cross reactions with phospho-serine or threonine. Tested on human breast cancer tissue.

ELISA (1:1000); WB (1:250); IP (10 µg/500 µg protein sample); IHC (1:50); Non-radioactive Tyrosine Kinase Assay

Tyrosine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to phospho-tyrosine which is considered a key step in the regulation of enzymatic activities such as signal transduction.

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.