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Product Description | The anti-phospho-tyrosine antibody is developed using phospho-tyrosine as the antigen and affinity-purified with phospho-tyrosine agarose. The purified antibody is immobilized to beaded agarose via strong thiol-ether linkages. This product could be utilized as an affinity matrix for rapid isolation and purification of proteins or peptides with phospho-tyrosine residues. | Immunoprecipitated samples with anti-phospho-tyrosine agarose (ICP9804). Left: Mouse spleen lysate treated with complete adjuvant. Right: Mouse spleen lysate without any treatment. Detection: Phospho-tyrosine antibody, HRP (ICP9902) | |
Formulation | 1 mL beaded agarose suspended in 2 mL slurry | ||
Antibody Immobilized | 5 mg/mL; antibody is covalently linked to CL-4B sepharose | ||
Specificity | The antibody selectively captures peptides or proteins with phosphorylated tyrosine residues. It does not cross react with non-phosphorylated proteins. | ||
Applications | Rapid isolation and purification of peptides or proteins with phospho-tyrosine residues from cell lysate or protease-digested mixtures. | ||
Scientific Description | Protein phosphorylation is one of the most important events in post-translational modification. Protein tyrosine phosphorylation has been extensively studied and is believed to be one of the switches of cell signaling. Simple and effective tools for rapid isolation and purification of the phosphorylated species is essential for proteomic profiling of the phosphorylated proteins in different stages of development, disease, and signal transduction, as well as identifying the precise site of phosphorylation in a phospho-protein. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. |
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化