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Product Description | The affinity purified rabbit polyclonal anti-Di-methylated lysine antibodies are developed using a technique unique to ImmuneChem. The Di-methylated lysine antibodies are affinity purified using N-Di-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The antibodies could be utilized for detection, quantization and isolation of proteins with Di-methylated lysine residues. | Western blot analysis of the di-methylated proteins from swine spleen cells lysate (lanes 4-5) and acid-extraction histones. (Lanes 2-3) with rabbit anti-di-methyl lysine antibodies (anti-MeK, ICP0701). | |
Species | Rabbit | ||
Formulation | 250 µg/mL in Tris-phosphate buffer, 50% glycerol | ||
Immunogen | Di-methylated KLH conjugates | ||
Purification | The antibody is immunoaffinity purified with epsilon N-Di-methyl lysine on agarose. | ||
Specificity | The antibody recognizes proteins or peptides with di-methyl lysine residues. There is no cross reaction with acetylated proteins. The cross reactivity with mono-methyl lysine has not been tested. | ||
Applications | ELISA,WB,IP | ||
Scientific Description | Protein lysine methylation (MeK) is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that di-methylation of lysine plays an important role in gene silencing.[1] | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is three years from date of shipping if stored properly. Do not aliquot. Shake thoroughly before use. | ||
Product Specific References |
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加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化