商品介绍
Methyl (mono) Arginine Antibody
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Product Description | This antibody is raised from rabbits that were immunized with NG- mono-methyl arginine-KLH conjugates and was specifically isolated and affinity purified using the monomethyl-arginine antigen. The affinity purified methyl arginine antibodies are useful for detection, isolation and localization of proteins with Nγ-methylated arginine residues. |  Competitive ELISA: inhibition of the binding mMeR-HRP to anti-mMeR antibody immobilized (1µg/mL) by free mMeR (IC50=40-50 ng/mL)
Western blot of human MMRU cells with ICP0801 (A,B) plus competitive inhibition with free methyl arginine (C,D)  Fluorescent Staining of MMRU cells | |
Species | Rabbit | ||
Formulation | 250 μg/mL in PBS, 50% glycerol | ||
Immunogen | NG-mono-methyl arginine-KLH conjugates | ||
Purification | Purified by antigen-specific affinity chromatography | ||
Specificity | The antibody recognizes free mono-methyl arginine and proteins with methylated arginine residues. It does not cross react with either asymmetric or symmetric dimethyl arginine. | ||
Species Tested | Pan-specific regardless of species. | ||
Applications | ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL) | ||
Scientific Description | Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from the date of shipping if properly stored. | ||
Product Specific References |
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品牌介绍
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供，并均已获得了技术持有人（公司）的授权。 主营产品公司提供以下产品： 1.细胞信号传导通路抗体，翻译后修饰抗体，细胞凋亡相关抗体，非放射性蛋白激酶测试盒，亲和填料； 2.磷酸化抗体，非磷酸化抗体，甲基化抗体，乙酰化
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