商品介绍
Methylated Lysine Antibody, Biotin
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Product Description | The affinity purified rabbit polyclonal anti-methylated lysine antibody is developed using a technique unique to ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The purified antibody was conjugated to d-biotin via acetylation. Direct labelling of the primary antibody will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody conjugates. |
Species | Rabbit |
Formulation | 250 µg/mL in PBS, 50% glycerol |
Immunogen | Methylated KLH conjugates |
Purification | Affinity purified with methyl-lysine and di-methyl-lysine on agarose |
Conjugation | Acetylation with NHS-biotin; Biotin/Ab molar ratio - 10:1 |
Specificity | The antibody recognizes anti-MeK (biotin conjugate) and recognizes proteins methylated on their lysine residues (mono and di-methyl lysine). It does not cross react with acetylated proteins. |
Applications | ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL) |
Scientific Description | MeK is a conserved post-translational modification which is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome B, chromosomal proteins, non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing. [1-3] |
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored. |
Product Specific References | 1.J. Biol. Chem. 2003.278:18346-18352.doi.10.1074/jbc.M300890200 2.J. Biol. Chem. 2002.277:34655-34657.doi:10.1074/jbc.C200433200 3.J. Biol. Chem.2002.277:11621-11624.doi:10.1074/jbc.C200045200 |
品牌介绍
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供，并均已获得了技术持有人（公司）的授权。 主营产品公司提供以下产品： 1.细胞信号传导通路抗体，翻译后修饰抗体，细胞凋亡相关抗体，非放射性蛋白激酶测试盒，亲和填料； 2.磷酸化抗体，非磷酸化抗体，甲基化抗体，乙酰化
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