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Product Description | Affinity purified rabbit polyclonal anti-methylated lysine antibody is developed using a methylated protein as the immunogen. The methyllysine specific antibodies are affinity purified with N-e-mono-methyllysine and di-methyllysine on agarose as the affinity matrix. The purified antibody was conjugated to fluorescein. The conjugated antibody was tested to localize methylated proteins. |
Species | Rabbit |
Formulation | 250 µg/mL in PBS, 50% glycerol |
Immunogen | Methylated KLH conjugates |
Purification | N-e-mono-methyllysine and di-methyllysine on agarose |
Specificity | Recognizes proteins methylated on lysine residues (mono and di-methyllysine). It does not cross react with acetylated proteins. The cross reactivity to tri-methyllysine has not been tested. |
Conjugation | With fluorescein isothiocyanate (FITC) FITC/Ab molar ratio - 10:1 |
Applications | IF (1:50 - 1:100) |
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is six months from date of shipping if properly stored. |
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化