商品介绍
Methylated Lysine Antibody, HRP
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Product Description | The affinity purified rabbit polyclonal anti-methylated lysine antibody was developed using a technique unique to ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The purified antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. Direct labelling of primary anti-MeK will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody-conjugates. The antibodies could be utilized for detection, quantization and isolation of proteins with MeK residues. |  Western blot analysis of the methylated protein pattern in whole HeLa cells with ICP0502. A. Signal of methylated proteins B. Signal of the methylated proteins was competitively suppressed by 50 µg/mL of methylated BSA C. Signal of the methylated proteins was competitively suppressed by 10 µg/mL of methylated BSA | |
Species | Rabbit | ||
Formulation | 500 µg/mL in HRP stabilizing buffer with 50% glycerol | ||
Immunogen | Methylated KLH conjugates | ||
Purification | Methyl-lysine and di-methyl lysine on agarose | ||
Conjugation | Reductive amination; HRP/Ab molar ratio - 10:1 | ||
Specificity | Anti-MeK peroxidase conjugates recognize proteins methylated on their lysine residues (mono and di-methyllysine). They do not cross react with acetylated proteins. | ||
Applications | ELISA; WB | ||
Scientific Description | MeK is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones and non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing. [1-3] | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly. | ||
Product Specific References | 1.J. Biol. Chem. 2003.278:18346-18352.doi.10.1074/jbc.M300890200 2.J. Biol. Chem. 2002.277:34655-34657.doi:10.1074/jbc.C200433200 3.J. Biol. Chem.2002.277:11621-11624.doi:10.1074/jbc.C200045200 |
品牌介绍
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供，并均已获得了技术持有人（公司）的授权。 主营产品公司提供以下产品： 1.细胞信号传导通路抗体，翻译后修饰抗体，细胞凋亡相关抗体，非放射性蛋白激酶测试盒，亲和填料； 2.磷酸化抗体，非磷酸化抗体，甲基化抗体，乙酰化
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