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Product Description | This antibody is raised from rabbits that were immunized with NG,NG-dimethyl arginine-KLH conjugates and specifically isolated and affinity purified using the NG,NG-dimethyl arginine antigen. The affinity purified methyl arginine antibody is useful for detection, isolation and localization of proteins with methylated arginine (asymmetric dimethylation) residues. | Antigen Structure | |
Species | Rabbit | ||
Formulation | 250 µg/mL in stabilizing buffer with 50% glycerol | ||
Immunogen | N5,NG-dimethyl arginine-KLH conjugates | ||
Conjugation | Aldehyde-activated HRP via reductive amination HRP/Ab ratio: 3:1 | ||
Specificity | The antibody recognizes proteins with dimethylated arginine residues. It does not cross react with acetylated proteins or mono-methylated proteins. | ||
Species Tested | Human, mouse and pig | ||
Applications | ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL) | ||
Scientific Description | Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified in such a way possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes including protein trafficking, signal transduction and transcriptional regulation. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly. | ||
Product Specific References | 1. Mol Microbiol.2007. 65: 590-606.doi:10.1111/j.1365-2958.2007.05831.x |
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化