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Product Description | Affinity purified rabbit polyclonal p44 MAPK antibody is developed using the C-terminal peptide of human MAPK (ERK1). | Western blot analysis of mouse brain extract (B) and spleen extract (A) with ICP9810. | |
Species | Rabbit | ||
Formulation | 250 µg/mL in TBS, 50% glycerol, 0.01% NaN3 | ||
Immunogen | Human p44 ERK1 C-terminal peptide-KLH conjugates | ||
Purification | The antibody was affinity purified with the p44 ERK1 C-terminal peptide on agarose. | ||
Specificity | The antibody recognizes p44 ERK1 of human, rat, bovine, mouse and rabbit. There is a slight cross reaction with p42 ERK2. | ||
Applications | ELISA; WB; IP | ||
Scientific Description | One of the members of mitogen-activated protein kinases is called P44 MAP Kinase (Erk1). It participates in cell growth and differentiation. [1,2] By phosphorylating transcription factors, (e., ELK-1), P44 MAP kinase regulates meiosis and mitosis of the cell. In the case of human MAP kinase, Erk1 activates Elk-1 through phosphorylation of threonine 202 and tyrosine 204. It is also known that Erk1 autophosphorylates tyrosine. [3] | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. | ||
Product Specific References | 1. Mol Cell Biol.1993.13: 4679–4690.doi:10.1128/MCB.13.8.46792. Cell.1995. 80: 179–185.doi:10.1016/0092-8674(95)90401-83. EMBO J. 1991. 10: 885–892. |
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化