商品介绍
Phospho-Crebtide Antibody, HRP
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Product Description | The affinity purified rabbit polyclonal anti-phospho-Crebtide (pCrebtide) antibodies are developed using a technique unique to ImmuneChem. The pCrebtide antibodies are affinity purified using phospho-Crebtide on agarose followed by affinity adsorption with the non-phospho-Crebtide. The purified antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. Direct labeling of the primary anti-pCrebtide will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody conjugates and also facilitating the assay. The antibodies could be utilized for the detection and quantization of phosphorylated Crebtide by target protein kinases. |
Species | Rabbit |
Formulation | 250 µg/mL in stabilizing buffer with 50% glycerol |
Immunogen | Phospho-crebtide (KRREILSRRPpSYR)-KLH |
Purification | Affinity purified with KRREILSRRPpSYR on agarose followed by affinity absorption with non-phosphorylated crebtide |
Conjugation | Reductive amination; HRP/Ab molar ration - 2:1 |
Specificity | The antibody only recognizes the phosphorylated crebtide product of PKA and PKC in a non-radioactive kinase assay. There are no cross reactions with non-phosphorylated crebtide. |
Applications | ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL) |
Scientific Description | Creb (cAMP response element binding) proteins are transcription factors that regulate certain activities of gene transcription. Crebtide is a peptide (KRREILSRRPSYR) derived from the Creb protein. PKC kinases phosphorylate crebtide to form phospho-crebtide. |
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is six months from date of shipping if properly stored. |
Product Specific References | 1. Cell. 1989.59(4): 675– 680.doi:10.1016/0092-8674(89)90013-5 |
品牌介绍
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供，并均已获得了技术持有人（公司）的授权。 主营产品公司提供以下产品： 1.细胞信号传导通路抗体，翻译后修饰抗体，细胞凋亡相关抗体，非放射性蛋白激酶测试盒，亲和填料； 2.磷酸化抗体，非磷酸化抗体，甲基化抗体，乙酰化
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