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Product Description | Affinity purified rabbit polyclonal anti-MBP antibody is developed with full length bovine myelin basic protein as the immunogen and affinity purified with an immuno-dominant MBP peptide. The peptide sequence is identical for most of the isoforms of mammalian myelin basic proteins. The purified antibody is conjugated to peroxidase (HRP) through reductive amination. The conjugates are tested for applications in sandwich ELISA detection of MBP in the plasma with an unoptimized sensitivity of 0.1 ng/mL, western-blot analysis and immunohistochemistry. | Detection of mouse MBP in a sandwich ELISA using ICP0495 | |
Species | Rabbit | ||
Formulation | 250 µg/mL in stabilizing buffer | ||
Immunogen | Bovine myelin basic protein (MBP) | ||
Purification | Affinity purified with synthetic MBP peptide on agarose | ||
Conjugation | Reductive amination with Ab/HRP ratio - 1:5 | ||
Specificity | Recognizes brain myelin basic proteins. Cross-reacts well with other tested animal species: mouse, swine, rat, chicken, human, dog, bovine and rabbits. May react with the MBP from other untested species too. | ||
Applications | ELISA (1:5000); WB (1:1000 - 1:2000); IHC (1:100 - 1:500) | ||
Storage & Stability | Product is stable at 4°C for 12 months. For extended storage, store product at -20°C. Expiration date for extended storage is six months from date of shipping if stored properly. |
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化