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Product Description | Chloramphenicol is covalently linked to HRP through acylation. Chloramphenicol-labelled HRP could be utilized for direct competitive ELISA for detection and quantization of chloramphenicol. | Direct competitive inhibition ELISA using immobilized anti-chloramphenicol (3 µg/mL), and chloramphenicol-labelled HRP (100 ng/mL). 50% inhibition occurred at 50 ng/mL of free chloramphenicol under non-optimized conditions. | |
Formulation | 1 mg/mL in PBS, 50% glycerol | ||
Conjugation | Chloramphenicol-succinyl-NHS active ester, via an acylation reaction Chloramphenicol/HRP molar ratio, approximately 3:1 | ||
Applications | ELISA | ||
Scientific Description | Chloramphenicol is a bacteriostatic antimicrobial agent originally derived from the bacterium Streptomyces venezuelae, isolated by David Gottlieb, and introduced into clinical practice in 1949. It was the first antibiotic to be manufactured synthetically on a large scale. Chloramphenicol is effective against a wide variety of microorganisms and is still very widely used in low income countries because it is exceedingly inexpensive. Detection and quantization of chloramphenicol in food (feed) is becoming common due to the issue of antibiotic abuse. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if properly stored. |
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化