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Product Description | This high affinity anti-Flg (DYKDDDDK) antibody is developed using the Flg peptide (DYKDDDDK) conjugated to KLH and affinity-purified with the DYKDDDDK peptide. The purified antibody was conjugated to peroxidase (HRP) via reductive amination. The antibody is useful for detection, isolation and localization of DYKDDDDK-tag proteins. | Western blot analysis of the cell extract with Flg-tag protein expression cell lysate ( 1 mg/mL) lane 2 = 25 µL; lane 3 = 15 µL; lane 4 = 10 µL cell lysate. Western blot analysis of the cell extract with Flg-tag protein expression (lane 7 = 25 µg; lane 8 = 5 µg; lane 9 = 10 µg cell lysate) | |
Species | Rabbit | ||
Formulation | 250 µg/mL in PBS, 50% glycerol | ||
Immunogen | Flg peptide (DYKDDDDK) KLH conjugates | ||
Conjugation | Reductive amination; HRP/Ab ratio - 2:1 | ||
Specificity | The antibody recognizes DYKDDDDK-tag proteins. | ||
Applications | ELISA (1:2500); WB (1 µg/mL) | ||
Scientific Description | DYKDDDDK peptide (Flag-tag) is frequently used as an affinity tag to facilitate the detection, isolation, and localization of the DYKDDDDK-fusion proteins. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly. |
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化