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Product Description | This high affinity anti-HA antibody is developed using HA peptide (YPYDVPDYA) conjugated to KLH and affinity-purified with the HA peptide. The antibody is useful for detection, isolation and localization of the HA-tag proteins. | Western-blot analysis of the HA-tag protein (50 kd) expressed cell lysate and affinity enriched by IP, with HA-tag antibody, HRP conjugates (ICP0751) 1: Molecular marker 2: 10 uL of HA-tag protein in lysate (original cell lysate) 3: 10 uL of His-tag protein in lysate (after IP depletion) 4: 10 uL of the HA-tag protein in 100 uL of the eluting buffer enriched with 25 ug of the anti-His tag (ICP0750) from 2 mL of the original lysate. Western blot analysis of the cell extract with expression of HA-tag proteins (B-F) and without expression of HA (A). | |
Species | Rabbit | ||
Formulation | 500 µg/mL in PBS, 50% glycerol | ||
Immunogen | GST peptide EEHLYERDEGDKWR KLH conjugates | ||
Purification | Antigen-specific affinity chromatography | ||
Specificity | The antibody recognizes HA-tag proteins. | ||
Applications | ELISA (1:2500); WB (1:1000); IP (5 µg/sample) | ||
Scientific Description | Hemaglutinin peptide (HA) is frequently used as an affinity tag to facilitate the detection, isolation and localization of the HA-fusion proteins. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C.Expiration date is one year from date of shipping if stored properly. | ||
Product Specific References | 1. Cancer Res.2010. 70: 10445.doi: 10.1158/0008-5472.CAN-10-3040 |
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化