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Product Description | This high affinity anti-HA antibody is developed using the HA peptide (YPYDVPDYA) conjugated to KLH and affinity-purified with the HA peptide. This antibody is conjugated to horse radish peroxidase (HRP) through reductive amination. Direct labelling of the primary antibody will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody-conjugates. | Western blot analysis of the HA-tag protein (50 kDa) expressed cell lysates with HA antibody HRP conjugates (ICP0751) 1: Marker2: 25 µL/lane 3: 15 µL/lane 4: 5 µL/lane 5: 2 µL/lane A BC Western blot analysis of the positive cell lysates with expression of HA-tag protein. A: MarkerB: 1 µL/lane C: 10 µL/lane | |
Species | Rabbit | ||
Formulation | 250 µg/mL antibody in peroxidase buffer | ||
Immunogen | Human influenza virus hemaglutinin (HA) peptide, (YPYDVPDYA) KLH conjugates | ||
Purification | Antigen-specific affinity chromatography | ||
Specificity | The antibody recognizes proteins fused with the (HA) peptide, YPYDVPDYA. | ||
Conjugation | Reductive amination; HRP/Ab molar ratio - 10:1 | ||
Applications | ELISA; WB; IP | ||
Scientific Description | Human influenza virus hemaglutinin peptide (HA) is frequently used as an affinity tag to facilitate the detection, isolation and localization of HA-fusion proteins. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly. |
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化