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Product Description | This high affinity his-tag antibody BIOTIN conjugate is developed using a polyhistidine pepide (HHHHHH) conjugated to KLH and affinity-purified with the HHHHHH peptide. The antibody is directly conjugated to d-Biotin and is useful for detection and localization of his-tag proteins. | ||
Species | Rabbit | ||
Formulation | 250 µg/mL in PBS, 50% glycerol | ||
Immunogen | Polyhistidine peptide (HHHHHH) KLH conjugates | ||
Conjugation | NHS-Biotin: Antibody molar ratio = 10:1 | ||
Purification | Antigen-specific affinity chromatography | ||
Specificity | The antibody recognizes his-tag proteins | ||
Applications | ELISA (1:2500); WB (0.25-1.0 ug/ml); IHC (5 ug/ml) | ||
Scientific Description | Polyhistidine peptide is frequently used as an affinity tag to facilitate the isolation of the 6H-fusion proteins using a metalchelate matrix. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Avoid cycles of freezing ang thawing. Expiration date is six months from date of shipping if stored properly. |
加拿大ImmuneChem Pharmaceuticals, Inc. www.immunechem.comImmuneChem是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有一流科学家和设备的实验室提供,并均已获得了技术持有人(公司)的授权。 主营产品公司提供以下产品: 1.细胞信号传导通路抗体,翻译后修饰抗体,细胞凋亡相关抗体,非放射性蛋白激酶测试盒,亲和填料; 2.磷酸化抗体,非磷酸化抗体,甲基化抗体,乙酰化